Functional Analysis

Using our industry leading know-how and our established in vitro analytical methods, Antibody Analytics can characterise and interrogate the biological function of your molecule.

Assays to Evaluate Direct Mechanism of Action:

  • All antibodies, or antibody-based, therapeutics will demonstrate a primary mechanism associated with the desired therapeutic function
  • These assays aim to characterise this biological effect
  • The antibody may act as an antagonist preventing a ligand-receptor interaction to inhibit an intracellular signalling pathway resulting in a reduced level of proliferation, or release of an inflammatory cytokine.

Assays Associated with the Effector Function of an Antibody bound to a Target Antigen:

  • Some antibodies target antigens expressed on the target cell surface, upon recognition by the complementary determining region of the antibody, effector cells/proteins recognise the Fc-region of the antibody
  • The types of receptor molecules that bind the Fc-region are inherent in the immune system, either proteins secreted into the blood stream, such as complement proteins, or expressed on the surface of lymphocytes. As a result of binding the Fc-region of the antibody, the antigen-expressing target cell is targeted for cytotoxicity.

Assays to investigate complex interactions:

  • These can be used to evaluate the biological activity of the candidate drug. These assays are generally exploratory, low-throughput and challenging in nature, provide a deeper understanding of the products therapeutic action.
  • Methods such as Mixed Lymphocyte Reaction (MLR), Trogocytosis, T-cell activation and differentiation