Exhausted
T CELL ASSAY

Test your candidates in a more physiologically relevant in vitro system

    Antibody Analytics have developed a model assay system which mimics the T cell status observed in cancer settings. Using our exhausted T cell model, we can help you determine how well your therapeutic candidates will perform in a more challenging system, where the T cells are phenotypically and functionally akin to those found in the tumor micro-environment.

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What is T cell exhaustion?

T cell exhaustion is a phenotypic and functional state of a T cell. It is characterised by increased cell surface expression of checkpoint inhibitors and a reduced functional capacity.

Exhausted T cells exhibit reduced proliferation, a reduction in cytokine production and function. In the presence of repeated antigenic stimulation in the context of chronic infection or cancer, increasing numbers of T cells reach this exhausted state and are less able to perform their intended anti-viral or anti-tumor functions.

Are you looking for other ways to better understand T cell interactions?

How does our Exhausted T cell model work?

1. T cells are isolated from PBMCs and are stimulated in culture via CD3 and CD28.

2. Repeated stimulations produce T cells which exhibit sustained up-regulation of multiple checkpoint inhibitors PD-1, TIM-3, LAG-3, CTLA-4, BTLA and the transcription factor TOX

3. These cells are not anergic but are comparatively less proliferative, cytotoxic and able to produce IFNγ and IL-2 than resting T cells

4. In the presence of your therapeutic candidate, the exhausted T cells can be plugged into a functional assay of your choice; T cell cytotoxicity, T cell activation or mixed lymphocyte reactions

Looking for a more technology based solution?

Other Assays you may be interested in
T Cell Mediated Cytotoxicity
Mixed Lymphocyte Reaction (MLR)
Cytokine Release Assay
T Reg
Suppression

If you feel our Exhausted T Cell Assay is the perfect environment to test your candidate in, get in touch. We have an expert Research and Development team that can customise the assay to suit the specific needs for each project.

We look forward to hearing from you.







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