T CELL ASSAY
Test your candidates in a more physiologically relevant in vitro system
- Antibody analytics have developed a model assay system which mimics the T cell status observed in cancer settings. Using our exhausted T cell model, we can help you determine how well your therapeutic candidates will perform in a more challenging system, where the T cells are phenotypically and functionally akin to those found in the tumor micro-environment.
Looking to find out more about how these work? Read on below
T cell exhaustion is a phenotypic and functional state of a T cell. It is characterised by increased cell surface expression of checkpoint inhibitors and a reduced functional capacity. Exhausted T cells exhibit reduced proliferation, a reduction in cytokine production and function. In the presence of repeated antigenic stimulation in the context of chronic infection or cancer, increasing numbers of T cells reach this exhausted state and are less able to perform their intended anti-viral or anti-tumor functions.
Are you looking for other ways to better understand T cell interactions?
EXHAUSTED T CELL MODEL
T cells are isolated from PBMCs and are stimulated in culture via CD3 and CD28
Repeated stimulations produce T cells which exhibit sustained up-regulation of multiple checkpoint inhibitors PD-1, TIM-3, LAG-3, CTLA-4, BTLA and the transcription factor TOX
These cells are not anergic but are comparatively less proliferative, cytotoxic and able to produce IFNγ and IL-2 than resting T cells
In the presence of your therapeutic candidate, the exhausted T cells can be plugged into a functional assay of your choice; T cell cytotoxicity, T cell activation or mixed lymphocyte reactions
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