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Test your candidates in a more physiologically relevant in vitro system

    Antibody Analytics have developed a model assay system which mimics the T cell status observed in cancer settings. Using our exhausted T cell model, we can help you determine how well your therapeutic candidates will perform in a more challenging system, where the T cells are phenotypically and functionally akin to those found in the tumor micro-environment.

Want to learn more about our Exhausted T Cell model?
Contact one of our team now to see how we can help

What is T cell Exhaustion?

T cell exhaustion is a phenotypic and functional state of a T cell. It is characterised by increased cell surface expression of checkpoint inhibitors and a reduced functional capacity.

Exhausted T cells exhibit reduced proliferation, a reduction in cytokine production and function. In the presence of repeated antigenic stimulation in the context of chronic infection or cancer, increasing numbers of T cells reach this exhausted state and are less able to perform their intended anti-viral or anti-tumor functions.

How does our Exhausted T cell model work?

1. T cells are isolated from PBMCs and are stimulated in culture via CD3 and CD28.

2. Repeated stimulations produce T cells which exhibit sustained up-regulation of multiple checkpoint inhibitors PD-1, TIM-3, LAG-3, CTLA-4, BTLA and the transcription factor TOX

3. These cells are not anergic but are comparatively less proliferative, cytotoxic and able to produce IFNγ and IL-2 than resting T cells

4. In the presence of your therapeutic candidate, the exhausted T cells can be plugged into a functional assay of your choice; T cell cytotoxicity, T cell activation or mixed lymphocyte reactions

Exhausted T Cell Assay

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Other Assays you may be interested in


In a results-driven manner, our expert immunologists can work with you to develop further immunological assays to interrogate the data arising from the cytokine release assay.


Our assay is readily adaptable to address your scientific questions. The T cell population to be suppressed can be selected from PBMCs, pan-T cells, CD4+ or CD8+ T cells as required.


MLR assays can take a variety of formats that should first be defined and optimised based on the requirements and the endpoints of the assay.


Our capabilities allow us to support early screening activities all the way through to the development of lot release potency assay methods.

Stay in touch with our R&D in this space and request further info