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Exhausted
T CELL ASSAY

Test your candidates in a more physiologically relevant in vitro system

    Antibody Analytics have developed a model assay system which mimics the T cell status observed in cancer settings. Using our exhausted T cell model, we can help you determine how well your therapeutic candidates will perform in a more challenging system, where the T cells are phenotypically and functionally akin to those found in the tumor micro-environment.

Ready to test your candidate in a more physiologically relevant in vitro system?
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What is T cell exhaustion?

T cell exhaustion is a phenotypic and functional state of a T cell. It is characterised by increased cell surface expression of checkpoint inhibitors and a reduced functional capacity.

Exhausted T cells exhibit reduced proliferation, a reduction in cytokine production and function. In the presence of repeated antigenic stimulation in the context of chronic infection or cancer, increasing numbers of T cells reach this exhausted state and are less able to perform their intended anti-viral or anti-tumor functions.

Are you looking for other ways to better understand T cell interactions?

How does our Exhausted T cell model work?

1. T cells are isolated from PBMCs and are stimulated in culture via CD3 and CD28.

2. Repeated stimulations produce T cells which exhibit sustained up-regulation of multiple checkpoint inhibitors PD-1, TIM-3, LAG-3, CTLA-4, BTLA and the transcription factor TOX

3. These cells are not anergic but are comparatively less proliferative, cytotoxic and able to produce IFNγ and IL-2 than resting T cells

4. In the presence of your therapeutic candidate, the exhausted T cells can be plugged into a functional assay of your choice; T cell cytotoxicity, T cell activation or mixed lymphocyte reactions

Looking for a more technology based solution?

Other Assays you may be interested in

CYTOKINE
RELEASE

In a results-driven manner, our expert immunologists can work with you to develop further immunological assays to interrogate the data arising from the cytokine release assay.

T REG
SUPPRESSION

Our assay is readily adaptable to address your scientific questions. The T cell population to be suppressed can be selected from PBMCs, pan-T cells, CD4+ or CD8+ T cells as required.

MIXED LYMPHOCYTE
REACTION (MLR)

MLR assays can take a variety of formats that should first be defined and optimised based on the requirements and the endpoints of the assay.

T CELL MEDIATED
CYTOTOXICITY

Our capabilities allow us to support early screening activities all the way through to the development of lot release potency assay methods.

Stay in touch with our R&D in this space and request further info