As shown in Figure 1, Literature values for the affinity of the TNFα - Remicade interaction range from ~5 - 20 pM. Therefore, the single concentration injection value of ~8 pM is highly accurate and can be further refined using our 2D kinetics approach with the mean affinity values inter-channel range from 6.8 pM to 9.0 pM.
In addition to accuracy, the optimised assay setup allows stronger confidence in your data with %CV values observed for ka (12%), kd (7%) and Rmax (3%). All well within regulatory guidelines.
Depending on the question you are looking to answer, we are able to tailor high throughput methods to suit your stage of development. Why not get in touch to discuss your requirements