T cell activation assays

T cell activation assays allow developers to rapidly identify T cell modulators

T cells play a critical role in cell-mediated immunity and can bring about long-term, antigen-specific, effector and memory responses. Scientists have developed a plethora of immunotherapy approaches aimed at inducing and engineering T cell responses, which have emerged as promising solutions for the treatment of disease like cancer. T cell activation is initiated by engagement of the TCR/CD3 complex, and the co-stimulatory receptor CD28. Co-stimulation of these receptors on the cell surface of T cells leads to intracellular signaling events.

Polyclonal approach

  • Our assay is based on stimulation of PBMCs with antibodies (αCD3/CD28), Staphylococcal enterotoxin B (SEB) or direct stimulation by recruitment of T cells to malignant target cells by a CD3-engager
  • Assay Readouts: Proliferation and activation markers by flow cytometry, gene expression, multiplex cytokine assay
  • Variations: Isolated T cells stimulated with αCD3/CD28
  • Proof of concept using Nivolumab, Dexamethasone, Methotrexate, Infliximab

Antigen specific approach

  • This is a more physiologically relevant activation of T cells relative to polyclonal
  • Assay of PBMCs with pathogen derived antigens or cancer antigens
  • Assay Readouts: Proliferation and activation markers by flow cytometry, gene expression, multiplex cytokine assay
  • Proof of concept using Nivolumab, Dexamethasone, Methotrexate, Infliximab